Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

6.9K Views

09:19 min

March 16th, 2020

DOI :

10.3791/60747-v

March 16th, 2020


Explore More Videos

GPCR

Chapters in this video

0:04

Introduction

0:45

Cell Membrane Solubilization and Protein Extraction

3:53

UV-VIS Spectroscopy

4:33

Automated Size-Exclusion Chromatography of Rhodopsin and Rhodopsin Mini-Go Complex

6:04

Deglycosylation

6:44

Results: UV-VIS Spectroscopy, SEC, and SDS-PAGE

8:34

Conclusion

Related Videos

article

10:37

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

14.8K Views

article

08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

29.6K Views

article

09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

10.3K Views

article

10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

8.1K Views

article

07:17

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

2.4K Views

article

06:18

High-Throughput Protein Crystallization via Microdialysis

2.0K Views

article

01:47

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

2.4K Views

article

01:42

Protein Sample Setup and Crystallization

562 Views

article

01:09

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

1.4K Views

article

07:08

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

2.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved