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Building a Simple and Versatile Illumination System for Optogenetic Experiments

DOI :

10.3791/61914-v

6:41 min

January 12th, 2021

January 12th, 2021

3,081 Views

1Department of Bioengineering, University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology, University of California, Irvine, 4Department of Biomedical Engineering, Duke University

This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

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Illumination System

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