JoVE Logo
Faculty Resource Center

Sign In

Building a Simple and Versatile Illumination System for Optogenetic Experiments (Video) | JoVE

DOI :

10.3791/61914-v

6:41 min

January 12th, 2021

January 12th, 2021

3,196 Views

1Department of Bioengineering, University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology, University of California, Irvine, 4Department of Biomedical Engineering, Duke University

This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

Tags

Illumination System

-- Views

Related Videos

article

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish (Video) | JoVE

article

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells (Video) | JoVE

article

Finite Element Modelling of a Cellular Electric Microenvironment (Video) | JoVE

article

Simple, Affordable, and Modular Patterning of Cells using DNA (Video) | JoVE

article

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells (Video) | JoVE

article

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications (Video) | JoVE

article

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans (Video) | JoVE

article

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation (Video) | JoVE

article

Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction (Video) | JoVE

article

Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved