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Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

DOI :

10.3791/54894-v

February 19th, 2017

February 19th, 2017

9,549 Views

1Department of Biomedical Engineering, University of Wisconsin-Madison

We designed a continuous culturing apparatus for use with optogenetic systems to illuminate cultures of microbes and regularly image cells in the effluent with an inverted microscope. The culturing, sampling, imaging, and image analysis are fully automated so that dynamic responses to illumination can be measured over several days.

Tags

Automated Illuminating System

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