CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
May 28th, 2019
•The CRISPR/Cas12a system in combination with a single crRNA array enables efficient multiplex editing of the S. cerevisiae genome at multiple loci simultaneously. This is demonstrated by constructing carotenoid producing yeast strains which are subsequently used to create yeast pixel art.
Related Videos
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Plasma Lithography Surface Patterning for Creation of Cell Networks
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved