Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo
3.3K Views
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10:35 min
•
February 5th, 2021
DOI :
February 5th, 2021
•Julia Volckmar1,2, Laura Knop1,2,3, Tatjana Hirsch1, Sarah Frentzel1,2, Christian Erck4, Marco van Ham4, Sabine Stegemann-Koniszewski*1,2,5, Dunja Bruder*1,2
1Immune Regulation Group, Helmholtz Centre for Infection Research, 2Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 3Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 4Cellular Proteome Research, Helmholtz Centre for Infection Research, 5Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg
Chapters in this video
0:04
Introduction
0:50
Anti-DEC-205 (CD205) Production
2:47
Anti-DEC-205 Antibody Purification
4:40
Chemical Conjugation
6:43
Chemical Conjugation Verification
8:22
Results: Representative Purified anti-DEC-205 Antibody Conjugation Characterization and Analysis
9:52
Conclusion
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