Antigenic Liposomes for Generation of Disease-specific Antibodies
12.3K Views
•
10:31 min
•
October 25th, 2018
DOI :
October 25th, 2018
•Kyle J. Bednar*1, Lakeya Hardy*2,3, Johanna Smeekens*3,4, Dharmendra Raghuwanshi5, Shiteng Duan6, Mike D. Kulis3,4, Matthew S. Macauley5,7
1Janssen R&D, 2Department of Microbiology and Immunology, University of North Carolina, 3UNC Food Allergy Initiative, University of North Carolina, 4Department of Pediatrics, University of North Carolina, 5Department of Chemistry, University of Alberta, 6Department of Molecular Medicine, Scripps Research Institute, 7Department of Medical Microbiology and Immunology, University of Alberta
Chapters in this video
0:04
Title
1:13
Protein Antigen-PEGylated Lipid Conjugation
2:57
Liposome Extrusion
4:24
Calcium Flux Monitoring of Antigenic Liposome-induced B Cell Activation
6:33
Mouse Peanut Sensitization and Ara h 2 (Ah2) Antigenic Liposome Challenge
8:26
Results: Representative Allergic Response Analyses
9:39
Conclusion
Related Videos
08:46
Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
3.7K Views
12:19
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
26.9K Views
10:33
Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
10.4K Views
09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
41.6K Views
08:29
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
10.0K Views
12:15
Generation of Monoclonal Antibodies Against Natural Products
10.8K Views
Tetrameric Fluorescent Antigen Arrays for Single-Step Identification of Antigen-Specific B Cells
1.8K Views
05:15
Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay
3.5K Views
01:29
Generating a Multivalent-Displaying Outer Membrane Vesicle Vaccine
45 Views
01:18
A Technique to Generate Monoclonal Antibodies from Antigen-Specific B Cells
146 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved