SARS-CoV-2 RNA-FIS in Vero Cells Cultured on Coverslips
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SARS-CoV-2 RNA-FISH in HAE Cultures
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Results: Immuno-RNA-FISH to Detect SARS-CoV-2 RNA in Vero and HAE Cells
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Conclusion
Transcript
This protocol made it possible to visualize SARS-CoV-2 RNA in cell lines and in fully differentiated, three-dimensional human airway epithelium cultures, providing a tool to better understand viral pathogenesis and replication at the single-cell l
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Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.