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A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

DOI :

10.3791/60660-v

7:20 min

February 28th, 2020

February 28th, 2020

9,288 Views

1Department of Surgery, University of Miami School of Medicine, 2Analytical Imaging Core Facility, University of Miami School of Medicine

A novel three-dimensional spheroid model based on the heterotypic interaction of tumor cells and stromal fibroblasts is established. Here, we present coculture of tumor cells and stromal fibroblasts, time-lapse imaging, and confocal microscopy to visualize the formation of spheroids. This three-dimensional model offers a pertinent platform to study tumor-stroma interactions and test cancer therapeutics.

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Novel Stromal Fibroblast Modulated 3D Tumor Spheroid Model

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