JoVE Logo
Faculty Resource Center

Sign In

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

DOI :

10.3791/62119-v

August 12th, 2021

August 12th, 2021

2,751 Views

1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

This fractionation protocol will allow researchers to isolate cytoplasmic, nuclear, mitochondrial, and membrane proteins from mammalian cells. The latter two subcellular fractions are further purified via isopycnic density gradient.

-- Views

Related Videos

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

article

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

article

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

article

Author Spotlight: A Novel Method for Comprehensive Cell Component Analysis of Cerebral Blood Clots

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved