February 26th, 2021
•Here, a protocol to isolate and transfect primary iris and retinal pigment epithelial cells from various mammals (mice, rat, rabbit, pig, and bovine) is presented. The method is ideally suited to study ocular gene therapy approaches in various set-ups for ex vivo analyses and in vivo studies transferable to humans.
Tags
Related Videos
Culture of myeloid dendritic cells from bone marrow precursors
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
Induction and Testing of Hypoxia in Cell Culture
Isolation and Primary Culture of Rat Hepatic Cells
Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved