Results: Isolation, Culture, and Transfection of Primary Pigment Epithelial (PE) Cells from Different Mammalian Species
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Conclusion
Transcript
The protocol is significant since it shows the isolation and transfection of pigment epithelial cells from five mammals as an optimal system to study ocular gene therapy in various setups. The main advantage of this method is its flexibility, tran
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Here, a protocol to isolate and transfect primary iris and retinal pigment epithelial cells from various mammals (mice, rat, rabbit, pig, and bovine) is presented. The method is ideally suited to study ocular gene therapy approaches in various set-ups for ex vivo analyses and in vivo studies transferable to humans.