JoVE Journal

Developmental Biology

This content is Open Access.

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Here we demonstrate an optimized technique for assessing wound repair using ex vivo human skin combined with a whole-mount staining approach. This methodology provides a pre-clinical platform for the evaluation of potential wound therapies.

Chapters in this video

0:05

Introduction

0:39

Preparation of Skin for Wounding and Creating Ex Vivo Human Skin Wounds

2:36

Whole Mount Staining of Ex Vivo Wounds

4:14

Explant Imaging

5:18

Results: Wound Closure Evaluation by Whole-Mount Tissue Staining

7:06

Conclusion

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