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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

DOI :

10.3791/62368-v

April 16th, 2021

April 16th, 2021

6,504 Views

1Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona

A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.

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