Mass Spectrometric Analysis and Lipid Class Identification and Quantification
6:53
Results: Representative Quantitative Analysis of Saccharomyces Cerevisiae Cellular Lipidomes
8:19
Conclusion
Transcript
This protocol provides an accurate and reproducible method for quantifying ten different lipid classes of Saccharomyces cerevisiae using a single extraction method and a single LC-MS method. This method allows the detection and quantification of i
Sign in or start your free trial to access this content
We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.