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Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

DOI :

10.3791/50757-v

9:18 min

September 5th, 2013

September 5th, 2013

20,714 Views

1Graduate Program of Environmental Science, Arkansas State University, 2Department of Biological Sciences, Arkansas State University, 3Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University

Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

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Triacylglyceride

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