Thin Layer Chromatography and Quantification of Results
7:09
Results: 14C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
8:03
Conclusion
Transcript
This technique uses instruments commonly found in most laboratory environments and allows for the analysis of several samples simultaneously. This method can provide key insights into how lipid metabolism enzymes are regulated in a cellular contex
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Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.