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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

DOI :

10.3791/62201-v

February 2nd, 2021

February 2nd, 2021

2,711 Views

1Department of Cell Biology, University of Texas Southwestern Medical Center

Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

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