Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
5.0K Views
•
09:49 min
•
March 16th, 2022
DOI :
March 16th, 2022
•Chapters in this video
0:04
Introduction
1:27
Grid Loading, Mapping, and Selection of Best Cryo-EM Grids for Data Collection
2:36
Setting up a Data Collection Session in the Single Particle Analysis Software
5:44
Final Microscope Alignments Before Starting Data Collection
7:15
Results: Gallery of Grids, Cryo-EM Reconstruction of Apo-Ferritin, and Effect of Energy Filtering on Cryo-EM Images
8:56
Conclusion
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