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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope Video (Video) | JoVE

DOI :

10.3791/63519-v

9:49 min

March 16th, 2022

March 16th, 2022

3,962 Views

1Materials and Structural Analysis Division, Thermo Fisher Scientific, 2Max Planck Institute of Biochemistry, Molecular Structural Biology, 3Materials and Structural Analysis Division, Thermo Fisher Scientific

High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.

Tags

Cryo EM

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