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Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

DOI :

10.3791/57197-v

9:47 min

July 17th, 2018

July 17th, 2018

8,702 Views

1National Institute for Physiological Sciences, 2Graduate School of Science and Engineering, Saitama University

This protocol describes how to visualize the transient DNA compaction in cyanobacteria. Synchronous cultivation, monitoring by fluorescence microscopy, rapid freezing, and high voltage cryo-electron tomography are used. A protocol for these methodologies is presented, and future applications and developments are discussed.

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DNA Compaction

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