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Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes

DOI :

10.3791/62432-v

7:10 min

May 7th, 2021

May 7th, 2021

3,670 Views

1Environmental Health Institute, National Environmental Agency

Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.

Tags

Fluorescent Dye

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