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Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

DOI :

10.3791/62502-v

9:43 min

June 13th, 2021

June 13th, 2021

1,639 Views

1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

Described here is an established method to determine the extent of HIV-1 restriction by the cellular inhibitory protein SAMHD1. Human myeloid lineage U937 cells are transduced with a SAMHD1 expression vector co-expressing YFP, differentiated and then challenged with HIV-RFP. The level of restriction is determined by flow cytometry analysis.

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SAMHD1 Restriction

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