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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

DOI :

10.3791/58139-v

8:10 min

July 28th, 2018

July 28th, 2018

11,462 Views

1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry

We provide a protocol for in vitro self-organization assays of MinD and MinE on a supported lipid bilayer in an open chamber. Additionally, we describe how to enclose the assay in lipid-clad PDMS microcompartments to mimic in vivo conditions by reaction confinement.

Tags

In Vitro Reconstitution

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