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Quantification of Coenzyme A in Cells and Tissues

DOI :

10.3791/60182-v

September 27th, 2019

September 27th, 2019

7,938 Views

1Department of Infectious Diseases, St. Jude Children's Research Hospital

This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.

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Coenzyme A

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