Extraction and Derivatization of CoA (Coenzyme A) in Cultured Cells
3:32
Extraction & Derivatization of CoA in Tissues
5:31
Sample Clean-up with Solid Phase Extraction Column
6:47
Results: HPLC Profiles and CoA-bimane Standard Curves
7:45
Conclusion
Transcript
Coenzyme A, called CoA for short, is an important cofactor in cellular metabolism. Quantitative measurements reveal changes that happen with nutritional and hormonal states in animal models. This method quantitates the total amount of cellular CoA
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This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.