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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

DOI :

10.3791/62651-v

July 6th, 2022

July 6th, 2022

4,575 Views

1Department of Pathology, Genentech, 2Department of Microchemistry, Proteomics, Lipidomics & Next Generation Sequencing (MPL-NGS), Genentech, 3NanoString Technologies

Here, we describe a protocol for fine-tuning regions of interest (ROIs) for Spatial Omics technologies to better characterize the tumor microenvironment and identify specific cell populations. For proteomics assays, automated customized protocols can guide ROI selection, while transcriptomics assays can be fine-tuned utilizing ROIs as small as 50 µm.

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