Automated Visualization Protocol for Spatial Proteomics Assays
6:02
Spatial Profiling Platform Protocol for Proteomics Assay
6:47
ROI Selection for Spatial Transcriptomics Assays
7:10
Results: Application of the Automated TSA Based Visualization Protocol in Combination with Spatial Proteomics Assays and Detection of Small ROIs in the Spatial Transcriptomics Protocol
8:47
Conclusion
Transcript
This protocol helps to guide the selection of regions of interest for spatial omics technologies, which allows a more targeted characterization of tissues or cell populations. An automated protocol for ROI selection in proteomics assays is more ro
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Here, we describe a protocol for fine-tuning regions of interest (ROIs) for Spatial Omics technologies to better characterize the tumor microenvironment and identify specific cell populations. For proteomics assays, automated customized protocols can guide ROI selection, while transcriptomics assays can be fine-tuned utilizing ROIs as small as 50 µm.