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Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level Video (Video) | JoVE

DOI :

10.3791/63307-v

March 14th, 2022

March 14th, 2022

2,667 Views

1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope

The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.

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