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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

DOI :

10.3791/62701-v

June 29th, 2021

June 29th, 2021

2,416 Views

1Department of Chemistry, Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University

We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.

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High pressure NMR Experiments

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