Sample Preparation and High-Pressure Cell Assembly
2:03
Recording High-Pressure NMR Experiments
2:32
Results: Unfolding of hnRNPA1 RRM2 Domain Under Pressure
4:11
Conclusion
Transcript
High pressure is the method of choice for changing the relative population of a protein conformational sample. It is extremely useful for isolating and characterizing high-energy conformational states. Compared to all the perturbation methods, lik
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We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.