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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

DOI :

10.3791/62723-v

August 11th, 2021

August 11th, 2021

2,278 Views

1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine, Washington State University

Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.

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Keywords Caenorhabditis Elegans

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