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Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

2.7K Views

08:31 min

June 30th, 2021

DOI :

10.3791/62768-v

June 30th, 2021


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Fluorescence Microscopy

Chapters in this video

0:05

Introduction

1:05

Tumor Cell Culture and Xenograft Tumor Development

2:41

HMWFD and NHF-ATP Solution Preparation

3:21

ATP Internalization in Tumor Xenograft

3:53

Cryo-embedding

4:45

Tissue Slide Fixation

5:25

Fluorescence Microscopy and Image Acquisition

7:12

Results: Assessment of Nonhydrolyzable Fluorescent ATP Internalization

7:44

Conclusion

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