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Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection

DOI :

10.3791/62831-v

August 27th, 2021

August 27th, 2021

2,106 Views

1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, 2National Plant Gene Research Center, 3University of the Chinese Academy of Sciences

The present protocol describes a method to collect sufficient saliva from piercing-sucking insects using an artificial medium. This is a convenient method for collecting insect saliva and studying salivary function on insect feeding behavior and vector-borne virus transmission.

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Keywords Membrane Sandwich Method

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