Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
7.5K Views
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08:32 min
•
February 14th, 2022
DOI :
February 14th, 2022
•Chi-Ju Kim*1,2, Morgan D. Kuczler*1, Liang Dong1,3, Junyoung Kim2,4, Sarah R. Amend1, Yoon-Kyoung Cho2,4, Kenneth J. Pienta1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)
Chapters in this video
0:04
Introduction
1:15
Extracellular Vesicle (EV) Isolation and On-Chip Immuno-Fluorescent Labeling
2:54
Incubation of the Cells with Fluorescently Labeled EVs for the EV-Uptake Assay
3:45
Confocal Microscopy and Image Processing
6:28
Results: EV Uptake Assay Using 3D Fluorescence Imaging via Confocal Microscopy
7:31
Conclusion
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