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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

DOI :

10.3791/62865-v

9:56 min

August 31st, 2021

August 31st, 2021

4,027 Views

1Neurophotonics and Mechanical Systems Biology, Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)

Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.

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Direct Force Measurements

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