Results: Determination of Initial Velocities of DNA Methylation in the Presence and Absence of Potential Inhibitors
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Conclusion
Transcript
A robust plate reader assay like ours is very useful for the initial screening of potential methyltransferase inhibitors. The ability to collect data in real time is a big advantage of the continuous endonuclease-coupled assay. Start by preparing
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DNA methyltransferases are potential cancer drug targets. Here, a protocol is presented to assess small molecules for DNA methyltransferase inhibition. This assay utilizes an endonuclease to couple DNA methylation to fluorescence generation and allows for enzyme activity to be monitored in real time.Â