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Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging (Video) | JoVE

DOI :

10.3791/63049-v

11:11 min

October 8th, 2021

October 8th, 2021

4,573 Views

1Department of Zoology and Physiology, University of Wyoming, 2School of Electrical Engineering & Computer Science, University of North Dakota, 3Department of Mathematics and Statistics, University of Wyoming

Miniscope in vivo calcium imaging is a powerful technique to study neuronal dynamics and microcircuits in freely behaving mice. This protocol describes performing brain surgeries to achieve good in vivo calcium imaging using a miniscope.

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Stereotaxic Viral Injection

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