A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease

5.3K Views

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07:17 min

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January 29th, 2021

DOI :

10.3791/62033-v

January 29th, 2021

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Vincent Law¹^,², Margi Baldwin³, Ganesan Ramamoorthi⁴, Krithika Kodumudi⁴, Nam Tran¹, Inna Smalley⁵, Derek Duckett⁶, Pawel Kalinski⁷, Brian Czerniecki⁴, Keiran S. M. Smalley², Peter A. Forsyth¹^,²

¹Department of Neuro-Oncology, H. Lee Moffitt Cancer Center & Research Institute, ²Department of Tumor Biology, H. Lee Moffitt Cancer Center & Research Institute, ³Department of Comparative Medicine, University of South Florida, ⁴Department of Breast Oncology, H. Lee Moffitt Cancer Center & Research Institute, ⁵Department of Cancer Physiology, H. Lee Moffitt Cancer Center & Research Institute, ⁶Department of Drug Discovery, H. Lee Moffitt Cancer Center & Research Institute, ⁷Department of Medical Oncology, Roswell Park Comprehensive Cancer Center

Summary

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Murine Ommaya Model

Leptomeningeal Disease

Targeted Therapy

Cerebrospinal Fluid Delivery

Central Nervous System Metastases

University Of South Florida

Buprenorphine Injection

Stereotactic Surgery

Surgical Technique

Cell Suspension Injection

Chapters in this video

0:04

Introduction

1:07

Injection of CTCs into CSF to Generate a Mouse LMD Model

3:37

Murine Ommaya Assembly and Implantation

5:20

Results: Direct-Targeted Immunotherapy Using the Murine Ommaya Increases the Overall Survival of Breast Cancer-Associated Leptomeningeal Disease Mice

6:37

Conclusion

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