Results: Metabolic and Physiologic Parameters of Isolated Perfused Newborn Murine Hearts
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Conclusion
Transcript
This approach reliably establishes retrograde perfusion of the neonatal ex vivo mouse heart in under three minutes. Thus reducing the youngest possible age of study from several weeks to 10 postnatal days. This isolated retrograde perfused model a
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The present protocol describes aortic cannulation and retrograde perfusion of the ex-vivo neonatal murine heart. A two-person strategy, using a dissecting microscope and a blunted small gauge needle, permits reliable cannulation. Quantification of longitudinal contractile tension is achieved using a force transducer connected to the apex of the left ventricle.