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Optical Mapping of Langendorff-perfused Rat Hearts

DOI :

10.3791/1138-v

August 11th, 2009

August 11th, 2009

20,426 Views

1Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, 2Departments of Cardiac Surgery, Children's Hospital Boston and Harvard Medical School

This article describes a high temporal and spatial resolution technique to optically image action potential movement on the surface of Langendorff-perfused rat hearts using a potentiometric dye (di-8-ANEPPS).

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