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Slicing the Embryonic Chicken Auditory Brainstem to Evaluate Tonotopic Gradients and Microcircuits

DOI :

10.3791/63476-v

8:24 min

July 12th, 2022

July 12th, 2022

1,715 Views

1Roxelyn and Richard Department of Communication Sciences and Disorders, Northwestern University, 2Knowles Hearing Research Center, Northwestern University, 3Department of Neurobiology, Northwestern University

Here we present a protocol for obtaining non-coronal auditory brainstem slices of the chicken embryo for the investigation of tonotopic properties and developmental trajectories within one brainstem slice. These slices include sagittal, horizontal, and horizontal/transverse sections encompassing larger tonotopic regions within an individual slice plane that the traditional coronal sections.

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Slicing

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