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In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

DOI :

10.3791/61664-v

August 18th, 2020

August 18th, 2020

5,242 Views

1Section on Neuronal Circuitry, National Institute on Deafness and Other Communication Disorders, NIH

Integration of diverse synaptic inputs to neurons is best measured in a preparation that preserves all pre-synaptic nuclei for natural timing and circuit plasticity, but brain slices typically sever many connections. We developed a modified brain slice to mimic in vivo circuit activity while maintaining in vitro experimentation capability.

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Keywords In Vitro

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