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Chicken Embryo Spinal Cord Slice Culture Protocol

DOI :

10.3791/50295-v

10:57 min

March 25th, 2013

March 25th, 2013

21,451 Views

1Research Department of Cell and Developmental Biology, University College London

Slice cultures facilitate the manipulation of embryo development by gene and pharmacological perturbations. However, culture conditions must ensure that normal development can proceed within the reduced environment of the slice. We illustrate a protocol that facilitates normal spinal cord development to proceed for at least 24 hr.

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Chicken Embryo

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