Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses
3.9K Views
•
07:56 min
•
June 8th, 2022
DOI :
June 8th, 2022
•Kittiwoot To-on1, Usana Chatturong1, Teerapap Panklai2, Iyapa Palang1, Anjaree Inchan1, Sutthinee Wisutthathum3, Tamkeen Urooj Paracha4, Phapada Apaikawee1, Krongkarn Chootip1
1Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry, Naresuan University, 2Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, 3Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences, Naresuan University, 4Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology, University of Calgary
Chapters in this video
0:05
Introduction
1:26
Isolation of Intrapulmonary Artery (IPA)
2:15
Isolation of Vascular Smooth Muscle Cells (VSMCs)
3:18
Organ Bath Technique
4:45
Vasorelaxant Response to Plant Extract
5:21
Mechanism of Plant Extract-Induced Vasorelaxation via the Endothelium
5:54
Results: Mechanistic Basis of the Vasorelaxant Action of the Plant Extract on Isolated Rat IPA and VSMCs
7:18
Conclusion
Related Videos
07:51
Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
28.5K Views
13:32
Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
10.7K Views
07:25
Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease
32.4K Views
09:23
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
15.6K Views
08:02
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
18.3K Views
08:24
Isolation of Murine Coronary Vascular Smooth Muscle Cells
14.1K Views
12:42
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
10.3K Views
08:52
Microdissection and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins
838 Views
08:28
Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
3.6K Views
08:59
Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
3.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved