JoVE Logo
Faculty Resource Center

Sign In

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

DOI :

10.3791/63974-v

10:10 min

July 20th, 2022

July 20th, 2022

2,563 Views

1Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU, Masaryk University, 3Department of Biochemistry, Faculty of Science, Masaryk University, 4Laboratory of Biomathematics, Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences

We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections.

Tags

Liver Stiffness

-- Views

Related Videos

article

An Antimicrobial Fabric Using Nano-Herbal Encapsulation of Essential Oils

article

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

article

Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Author Spotlight: Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

article

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

article

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved