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Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI

DOI :

10.3791/3362-v

January 30th, 2012

January 30th, 2012

16,736 Views

1Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center, College of Medicine

We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.

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