Assay for Adhesion and Agar Invasion in S. cerevisiae

Take 200 microliters of your culture of interest And just make a goal. I can usually Fit up to six in month plate if I want to, and then I leave, it will open And let it dry. And these are two plates.

One of them has mold in it, but it's fine because it doesn't affect our experiment. And one of them is in complete, one of them is in low glucose medium. And so we're gonna look at adhesion and invasion and see if there's a difference.

So just take the plate out like that. And you can see stuff growing. I'm gonna wash.

You can see the bubbles under the auger, and that's because water got under the auger, which is fine, but just so that the auger doesn't slip out of the plate. So I would say that even though some cells came off, both of these strains are adhesive. The cells are adhering to other cells that are adhering perhaps to the agar.

Otherwise a lot of the cells would come off and they didn't. So, so now that we've documented adhesion, we're gonna document invasion. I'm gonna rub the cells off from the surface gently, as much as they come off.

I'm not putting any pressure, but I'm trying to get anything that will come off of the other surface. And you'll see, you'll feel your finger will bump where the calling is Trying, I'm trying to dry it. Can we see the difference here?

You can't, which is good because this is a complete plate and so they're not starving for anything. And this is a low glucose plate and they're starving for glucose. So then you see a difference.

So that's a good control.