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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

DOI :

10.3791/62061-v

January 5th, 2021

January 5th, 2021

3,659 Views

1Department of Biology, Concordia University, 2Centre for Biological Applications of Mass Spectrometry, Concordia University

We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.

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