Results: Representative Metabolic Intermediate and Product Identification and Quantification
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Conclusion
Transcript
This protocol has many advantages over the currently used yeast metabolomics because it has high specificity, high sensitivity, and also it can profile many different classes of metabolites, including isomeric, isobaric, hydrophilic, and hydrophob
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We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.