Microfluidic Channel Fabrication and Pre‐Treatment
1:49
Cell Culture in the Microfluidic Channel
2:35
Cell Fixation
4:11
Cell Staining and Mounting
5:25
Cell Imaging with Confocal Microscopy
5:55
Results: Culture Duration Effects on Monolayer Production
6:52
Conclusion
Transcript
This protocol serves to lower a barrier to entry for cell culture experimentation and enables researchers to evaluate and characterize adherent cell monolayers cultured in dynamic microfluidic environments. The primary advantage of this technique
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The presented protocol describes the development and use of a phalloidin-based filamentous-actin staining technique with confocal laser scanning microscopy (CLSM) to visualize adherent cell layer structure in microfluidic dynamic-culture channels and traditional fixed-well static-culture chambers. This approach aids in evaluating cell layer confluency, monolayer formation, and layer-thickness uniformity.