July 1st, 2022
•The presented protocol describes the development and use of a phalloidin-based filamentous-actin staining technique with confocal laser scanning microscopy (CLSM) to visualize adherent cell layer structure in microfluidic dynamic-culture channels and traditional fixed-well static-culture chambers. This approach aids in evaluating cell layer confluency, monolayer formation, and layer-thickness uniformity.
Tags
Related Videos
Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
A Microfluidic Device for Studying Multiple Distinct Strains
Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved