An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

2.9K Views

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11:04 min

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January 13th, 2023

DOI :

10.3791/64391-v

January 13th, 2023

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Isadora da Silva Zanzarini¹, Gustavo Barbosa¹, Larissa de Oliveira Prado¹, Ingrid Fatima Zattoni¹, Gustavo Da Paz¹, Ademir Luiz do Prado²^,³, Waldemar Volanski², Marcos Dinís Lavarda³, Fabiane Gomes de Moraes Rego², Geraldo Picheth², Vivian Rotuno Moure¹, Glaucio Valdameri¹

¹Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance, Federal University of Parana, ²Graduate Program in Pharmaceutical Sciences, Federal University of Parana, ³Federal Institute of Parana

Summary

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Photodynamic Therapy

PDT

Light emitting Diode

Verteporfin Cytotoxicity

Dulbecco s Modified Eagle Medium

Low Glucose

Chapters in this video

0:02

Introduction

0:40

Device Construction

3:10

Cell Lines: Cultivation, Seeding, and Treatment

5:19

Device Operation

6:56

Cell Viability Assays

7:47

Representative Results

9:50

Conclusion

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