Isolation of T Cells with Microbubbles Using Positive Selection
1:51
Co-Stimulation (Activation) of the Positively Selected T Cells
2:52
Expansion of the Co-Stimulated Cells in Cell Culture Medium
3:28
Expansion of the T Cells (With or Without Prior Transduction)
4:02
Harvesting the T Cells and Flow Cytometry
5:16
Results: Assessment of Viable and Successfully Transduced Cells
5:58
Conclusion
Transcript
The microbubble activation and expansion protocol is significant because it addresses a major unmet need in cell therapy research and manufacturing, improving the persistence and efficacy of T-cell therapies. Akadeum's buoyancy-based positive sele
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The objective of this study is to demonstrate the feasibility of flotation-based separation to isolate, activate, and expand primary human T cells.