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A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

DOI :

10.3791/64641-v

6:18 min

March 3rd, 2023

March 3rd, 2023

723 Views

1Harris Orthopaedics Laboratory, Massachusetts General Hospital, 2Department of Orthopaedic Surgery, Harvard Medical School

Here, we present a protocol to evaluate the antibacterial efficacy of an antibiotic-eluting polymer to simulate prophylactic clinical application by using a commercially available real-time ATP-based luminescent microbial viability assay. This method enables the monitoring of the longitudinal activity of drug-eluting materials and can be widely adapted to test anti-microbial drug delivery platforms.

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Antibacterial Activity

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