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Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

DOI :

10.3791/64550-v

12:29 min

March 24th, 2023

March 24th, 2023

891 Views

1Bacterial Genetics and Physiology, Département de Biologie Moléculaire, Faculté des Sciences, Université Libre de Bruxelles (ULB)

Antibiotic persistence describes the ability of small subpopulations within a sensitive isogenic population to transiently tolerate high doses of bactericidal antibiotics. The present protocol combines approaches to characterize the antibiotic persistence phenotype at the molecular and cellular levels after exposing Escherichia coli to lethal doses of ofloxacin.

Tags

Population Analysis

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