Name: model of ischemia and reperfusion injury in rabbits
Uploaded: 2023-11-03T13:40:00
Description: Watch this Scientific Journal Video about Model of Ischemia and Reperfusion Injury in Rabbits at JoVE.com

使用该方案的原始研究得到了美国国家心脏、肺和血液研究所资助 HL-103642 和 HL-088206 的支持

这项调查是根据美国国立卫生研究院的动物护理和使用指南进行的，并得到了波士顿儿童医院动物护理和使用委员会的批准（协议 20-08-4247R）。所有动物均按照《实验动物护理和使用指南》的规定得到人道照顾。
1.动物种类、麻醉剂和镇痛剂
<ol><li>动物种类：使用新西兰白兔（野生型品系;雌性;性成熟15-20周龄;体重3-4公斤）进行实验研究。</li>
	<li>麻醉和镇痛剂?...

<ol>
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E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+acute+regional+myocardial+ischemia+and+reperfusion+in+the+rat.">A model of acute regional myocardial ischemia and reperfusion in the rat.</a> Magnetic Resonance in Medicine. 10 (2), 172-181 (1989).</li><li>Rodr&#237;guez, B., Trayanova, N., Noble, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modeling+cardiac+ischemia.">Modeling cardiac ischemia.</a> Annals of the New York Academy of Sciences. 1080, 395-414 (2006).</li><li>Sinusas, A. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+area+at+risk+during+coronary+occlusion+and+degree+of+myocardial+salvage+after+reperfusion+with+technetium-99m+methoxyisobutyl+isonitrile.">Quantification of area at risk during coronary occlusion and degree of myocardial salvage after reperfusion with technetium-99m methoxyisobutyl isonitrile.</a> Circulation. 82 (4), 1424-1437 (1990).</li><li>Masuzawa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+autologously+derived+mitochondria+protects+the+heart+from+ischemia-reperfusion+injury.">Transplantation of autologously derived mitochondria protects the heart from ischemia-reperfusion injury.</a> American Journal of Physiology. Heart and Circulatory Physiology. 304 (7), H966-H982 (2013).</li><li>Pombo, J. F., Troy, B. L., Russell, R. O. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Left+ventricular+volumes+and+ejection+fraction+by+echocardiography.">Left ventricular volumes and ejection fraction by echocardiography.</a> Circulation. 43 (4), 480-490 (1971).</li><li>McCully, J. D., Wakiyama, H., Hsieh, Y. J., Jones, M., Levitsky, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+contribution+of+necrosis+and+apoptosis+in+myocardial+ischemia-reperfusion+injury.">Differential contribution of necrosis and apoptosis in myocardial ischemia-reperfusion injury.</a> American Journal of Physiology. 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M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+myocardial+and+vascular+injury.">Animal models of myocardial and vascular injury.</a> Journal of Surgical Research. 162 (2), 239-249 (2010).</li><li>Pirat, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+feature-tracking+echocardiographic+method+for+the+quantitation+of+regional+myocardial+function:+Validation+in+an+animal+model+of+ischemia-reperfusion.">A novel feature-tracking echocardiographic method for the quantitation of regional myocardial function: Validation in an animal model of ischemia-reperfusion.</a> Journal of the American College of Cardiology. 51 (6), 651-659 (2008).</li><li>Verdouw, P. D., vanden Doel, M. A., de Zeeuw, S., Duncker, D. 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我们的方案展示了一种在兔子中进行急性局部心肌缺血的可靠方法。左侧微型开胸手术方法非常适合生存病例，必须尽量减少切口和相关疼痛。重要的是，拔管前不需要利尿剂治疗，非生存组术中或术后 4 周时没有死亡率。当方案的设计需要非生存病例时，或者当需要更详细地监测整体和区域心肌功能时，可以使用中线胸骨切开术（图 1）。
该方案最关...

心血管疾病是世界上的主要死亡原因，每年导致超过1800万人死亡1,2,3。急性心肌梗死 （MI） 是一种常见的医疗急症，当血凝块或一块动脉粥样硬化斑块阻塞冠状动脉的血流时就会发生。这会导致动脉灌注区域的局部心肌缺血。
本研究描述了一种方案，该方案利用一种简单可靠的方法在兔模型中创建原位急性区域心肌缺血，用于非生存和生存实验。该方法的最初目标是评估线粒体移植对调节心肌坏死和增加缺血事件后缺血后心脏功能的影响。先前的研究表明，在缺血发作和氧气供应减少后，线粒体改变和高能磷酸盐水平迅速下降，导致心脏能量储存急剧下降4.研究人员试图使用药物干预和/或程序技术来改善缺血后功能并减轻心肌组织坏死，但这些技术提供的心脏保护有限，对线粒体损伤和功能障碍的影响最小 5,6,7。我们的团队和其他人之前已经表明，线粒体损伤主要发生在缺血期间，并且随着再灌注期间线粒体呼吸功能的保留，收缩恢复可以增强，心肌梗死面积减小 8,9,10。因此，我们假设线粒体从未受缺血影响的组织移植到再灌注前的缺血区域将提供一种减少心肌坏死和增强心肌功能的替代方法。在此，我们详细介绍了用于测试该理论的协议以及从我们的初步研究分析中获得的代表性结果。
此外，一些研究人员还关注了其他主题，这些主题对于确定心肌缺血再灌注损伤的影响和建立适当的治疗干预措施至关重要。其中一个研究领域是预处理。心肌缺血预处理是一种由短暂缺血应激激活的心脏保护机制，可在随后的长期缺血发作期间降低心肌细胞坏死率。这些机制可以通过缺氧或冠状动脉闭塞激活。Mandel 等人证明，缺氧-高氧预处理有助于维持一氧化氮代谢物的平衡，减少内皮素-1 的过度产生，并支持器官保护11。此外，还探讨了远程缺血预处理的概念，这是一种单器官预处理提供全身保护的现象。Ali 等人发现，在接受择期开腹主动脉瘤修复术的患者中，通过间歇叉夹住髂总动脉作为刺激进行远程预处理可降低术后心肌损伤、心肌梗死和肾功能损害的发生率12。
与其他物种的模型相比，兔子模型具有潜在的优势，并且几十年来一直用于多种不同的场景，包括心律失常的诱导、全球和区域缺血模型以及心脏收缩研究等13,14,15。虽然兔子的心脏比狗或猪的心脏小，但它足够大，可以以更低的成本轻松进行外科手术13.兔子心脏经常被使用，因为它与人类的心脏非常相似;事实上，它具有相似的代谢率，表达β-肌球蛋白重链，并且缺乏显着的心肌黄嘌呤氧化酶16。本文所描述的诱导局部心肌缺血的技术是简单、可重复且具有成本效益的。这种方法允许非生存和生存病例，因为仅诱导局部缺血而不是全局缺血，并且所需的材料是非特异性的。可以使用两种不同的手术方法（即胸骨切开术和微型开胸术），从而在研究设计方面为操作员和实验方案提供了更大的自由度。此外，该手术不需要使用体外循环。在这种情况下，冠状动脉旁路移植术的微创方法已成为需要多血管血运重建的患者的宝贵替代方案17,18。该模型可用于研究这些方法之间的差异，并为外科实习生提供基于动物的学习工具。此外，利用该模型进行心导管插入术可能有助于生理学研究和/或外科训练。
我们的模型为诱导区域心肌缺血并随后测量梗死大小、心肌功能和细胞变化的应用提供了一种方法。通过该协议，我们已经能够通过检查细胞器的内化、耗氧量、高能磷酸盐合成以及细胞因子介质和蛋白质组学途径的诱导来评估细胞功能和对缺血的适应以及拟议的治疗干预（即线粒体移植）的几个标志物。这些结果对于保持心肌能量、细胞活力和心脏功能非常重要，并允许客观评估缺血再灌注损伤后的心脏保护技术。该模型可用于研究缺血后心肌病理学和恢复领域的类似生物学途径和替代方案。
该方案的目标是提供一种高度可重复的方法，以诱导兔子原位急性局部心肌缺血，用于非生存和生存实验。该模型提供了一种高生存率、低术中死亡率和最低发病率的方法19。已经使用放射性标记材料、造影剂、磁共振成像或计算机模拟描述了急性区域心肌缺血的其他模型20,21,22。我们的方案提供了一种可靠且简单的方法，该方法具有成本效益，始终如一的可重复性，并且技术要求低，因此可以由没有手术专业知识的研究人员执行。该协议适用于使用左侧迷你开胸术的生存项目或使用中线胸骨切开术的非生存模型。

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			<td>Bard Parker</td>
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			<td>#11 blade</td>
			<td>Fisher Scientific</td>
			<td>B3L</td>
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			<td>22 G PIV needle</td>
			<td>BD Insyte</td>
			<td>381423</td>
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			<td>VETONE</td>
			<td>NDC 13985-587-50</td>
			<td>0.5 mg/kg IM and IV</td>
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			<td>Aline pressure bag</td>
			<td>Infu-Stat</td>
			<td>2139</td>
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			<td>Becton Dickinson</td>
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			<td>Arterial Catheter</td>
			<td>Teleflex</td>
			<td>MC-004912</td>
			<td></td>
		</tr>
		<tr>
			<td>Atropine</td>
			<td>Hikma Pharmaceuticals</td>
			<td>NDC 0641-6006-01</td>
			<td>&#160;0.01 mg/kg IM</td>
		</tr>
		<tr>
			<td>Betadine and 70% isopropyl alcohol</td>
			<td>McKesson</td>
			<td>NDC 68599-2302-6</td>
			<td></td>
		</tr>
		<tr>
			<td>Blood gas machine</td>
			<td>Siemens</td>
			<td>MRK0025</td>
			<td></td>
		</tr>
		<tr>
			<td>Bovie</td>
			<td>Valleylab</td>
			<td>E6008</td>
			<td></td>
		</tr>
		<tr>
			<td>Bulldog clamps</td>
			<td>World Precision Instruments</td>
			<td>14119</td>
			<td></td>
		</tr>
		<tr>
			<td>Bupivacaine</td>
			<td>Auromedics</td>
			<td>NDC 55150-249-50</td>
			<td>&#160;3 mg/kg IM</td>
		</tr>
		<tr>
			<td>Butorphanol</td>
			<td>Roxane</td>
			<td>NDC 2054-3090-36</td>
			<td>0.5 mg/kg IM</td>
		</tr>
		<tr>
			<td>Clear acetate sheet</td>
			<td>Oxford Instruments</td>
			<td>ID 51-1625-0213</td>
			<td></td>
		</tr>
		<tr>
			<td>Clipers</td>
			<td>Andis</td>
			<td>AGC2</td>
			<td></td>
		</tr>
		<tr>
			<td>DeBakey forceps</td>
			<td>Integra</td>
			<td>P6280</td>
			<td></td>
		</tr>
		<tr>
			<td>Echocardiography machine</td>
			<td>Philips</td>
			<td>IE33 F1</td>
			<td></td>
		</tr>
		<tr>
			<td>Electrocardiography machine</td>
			<td>Meditech</td>
			<td>MD908B</td>
			<td></td>
		</tr>
		<tr>
			<td>Endotracheal tube</td>
			<td>Medline</td>
			<td>#922774</td>
			<td></td>
		</tr>
		<tr>
			<td>Fentanyl</td>
			<td>West-Ward</td>
			<td>NDC 0641-6030-01</td>
			<td>1&#8211;4 &#181;g/kg transdermal patch</td>
		</tr>
		<tr>
			<td>Formaldehyde solution 10%</td>
			<td>Epredia</td>
			<td>94001</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass plates</td>
			<td>&#160;United Scientific</td>
			<td>B01MUHX6MR</td>
			<td></td>
		</tr>
		<tr>
			<td>Heparin Sodium</td>
			<td>Sagent</td>
			<td>NDC 69-0058-02</td>
			<td>1000U in 1 mL 3 mg/kg</td>
		</tr>
		<tr>
			<td>Hot water blanket</td>
			<td>3M</td>
			<td>55577</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Penn Veterinary Supply, INC</td>
			<td>NDC 50989-606-15</td>
			<td>1%&#8211;3%</td>
		</tr>
		<tr>
			<td>Ketamine</td>
			<td>Dechra</td>
			<td>NDC 42023-138-10</td>
			<td>10 mg/kg IV</td>
		</tr>
		<tr>
			<td>Lab Chart 7 Acquisition Software</td>
			<td>Adinstruments</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Lactated Ringer&#39;s solution</td>
			<td>ICUmedical</td>
			<td>NDC 0990-7953-09</td>
			<td>10 mL/kg/h</td>
		</tr>
		<tr>
			<td>Laryngoscope</td>
			<td>Welch Allyn</td>
			<td>68044</td>
			<td></td>
		</tr>
		<tr>
			<td>Left ventricule lumen catheter&#160;3Fr</td>
			<td>McKesson</td>
			<td>385764-EA</td>
			<td></td>
		</tr>
		<tr>
			<td>Lidocaine (1%)</td>
			<td>Pfizer</td>
			<td>4276-01</td>
			<td>1&#8211;1.5 mL/kg IV</td>
		</tr>
		<tr>
			<td>LVDP transducer</td>
			<td>Edward</td>
			<td>PDP-ED</td>
			<td></td>
		</tr>
		<tr>
			<td>Marking pen</td>
			<td>Viscot</td>
			<td>1451SR-100 Unsterile</td>
			<td></td>
		</tr>
		<tr>
			<td>Mayo scissors</td>
			<td>Mayo</td>
			<td>S7-1098</td>
			<td></td>
		</tr>
		<tr>
			<td>Medetomidine</td>
			<td>Entireoly Pets Pharmacy</td>
			<td>NDC 015914-005-01</td>
			<td>0.25 mg/kg IM</td>
		</tr>
		<tr>
			<td>Metzenbaum scissors</td>
			<td>Cole-Parmer</td>
			<td>UX-10821-05</td>
			<td></td>
		</tr>
		<tr>
			<td>Monastra. Blue pigment 98%</td>
			<td>Chemsavers</td>
			<td>MBTR1100G</td>
			<td></td>
		</tr>
		<tr>
			<td>Monocryl 5-0</td>
			<td>Ethicon</td>
			<td>Y463G</td>
			<td></td>
		</tr>
		<tr>
			<td>Mosquito clamp</td>
			<td>Shioda</td>
			<td>802N</td>
			<td></td>
		</tr>
		<tr>
			<td>PDS 3-0</td>
			<td>Ethicon</td>
			<td>42312201</td>
			<td></td>
		</tr>
		<tr>
			<td>Piezoelectric sonomicrometry crystals</td>
			<td>Sonometrics</td>
			<td>Small 2mm round</td>
			<td></td>
		</tr>
		<tr>
			<td>Plegets</td>
			<td>DeRoyal</td>
			<td>32-363</td>
			<td></td>
		</tr>
		<tr>
			<td>Povuine Iodine Prep Solutions</td>
			<td>Medline</td>
			<td>MDS093940</td>
			<td></td>
		</tr>
		<tr>
			<td>Precision vaporized system face mask</td>
			<td>Yuwell</td>
			<td>B07PNH69BF</td>
			<td></td>
		</tr>
		<tr>
			<td>Prolene 3-0</td>
			<td>Ethicon</td>
			<td>8665G</td>
			<td></td>
		</tr>
		<tr>
			<td>Proline 5-0</td>
			<td>Ethicon</td>
			<td>8661G</td>
			<td></td>
		</tr>
		<tr>
			<td>Pulse oximetry probe</td>
			<td>Masimo</td>
			<td>9216-U</td>
			<td></td>
		</tr>
		<tr>
			<td>Rib spreader</td>
			<td>Medline</td>
			<td>MDS5621025</td>
			<td></td>
		</tr>
		<tr>
			<td>S12 Pediatric Sector Probe</td>
			<td>Phillips</td>
			<td>21380A</td>
			<td></td>
		</tr>
		<tr>
			<td>Sonomicrometer</td>
			<td>Sonometrics</td>
			<td>BZ10123724</td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile gauze</td>
			<td>Medline</td>
			<td>3.00802E+13</td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile towels</td>
			<td>McKesson</td>
			<td>MON 277860EA</td>
			<td></td>
		</tr>
		<tr>
			<td>Sternal retractor</td>
			<td>Medline</td>
			<td>MDS5610321</td>
			<td></td>
		</tr>
		<tr>
			<td>Sutures for closure</td>
			<td>J&#38;J Dental</td>
			<td>8698G</td>
			<td></td>
		</tr>
		<tr>
			<td>Telemetriy monitor</td>
			<td>Meditech</td>
			<td>MD908B</td>
			<td></td>
		</tr>
		<tr>
			<td>Temperature probe</td>
			<td>Omega</td>
			<td>KHSS-116G-RSC-12</td>
			<td></td>
		</tr>
		<tr>
			<td>Triphenyl tetrazolium chloride (1%)</td>
			<td>Millipore</td>
			<td>MFCD00011963</td>
			<td></td>
		</tr>
		<tr>
			<td>Ventilator</td>
			<td>MedGroup</td>
			<td>MSLGA 11</td>
			<td></td>
		</tr>
		<tr>
			<td>Vicryl 2-0</td>
			<td>Ethicon</td>
			<td>V635H</td>
			<td></td>
		</tr>
		<tr>
			<td>Vinyl tubing</td>
			<td>ABE</td>
			<td>DISW 3001</td>
			<td></td>
		</tr>
	</tbody>
</table>

model of ischemia and reperfusion injury in rabbits

这里的方案提供了一种简单、高度可重复的方法，用于非生存和生存实验在兔子中诱导原 位 急性区域心肌缺血。新西兰白成年兔用阿托品、乙酰丙嗪、布托啡醇和异氟烷镇静。对动物进行插管并置于机械通气状态。将静脉导管插入边缘耳静脉以输注药物。用肝素、利多卡因和乳酸林格氏液预先给动物用药。进行颈动脉切断术以获得动脉管路以监测血压。通过连续实时分析监测和记录选定的生理和机械参数。
在动物镇静并完全麻醉的情况下，进行第四个肋间小左开胸术（存活）或中线胸骨切开术（非存活）。打开心包，找到左前降支 （LAD）。
聚丙烯缝合线绕过LAD动脉的第二或第三对角线分支，聚丙烯长丝穿过一个小乙烯基管，形成圈套。动物经受30分钟的局部缺血，通过收紧圈套来闭塞LAD。心肌缺血可通过心外膜局部发绀在视觉上确诊。局部缺血后，结扎松动，心脏重新灌注。
对于生存和非生存实验，都 可以通过 超声心动图 （ECHO） 测量缩短分数来评估心肌功能。对于非生存研究，可以使用植入缺血区域的三个数字压电超声探头和使用根尖插入的左心室 （LV） 导管的左心室发育压力 （LVDP） 收集的超声显微测量数据可以连续获取，分别用于评估区域和整体心肌功能。
对于生存研究，切口闭合，进行左针胸腔穿刺术以清除胸腔空气，并实现术后疼痛控制。

按照方案（图1），通过心外膜紫绀的直接可视化立即确认心肌缺血。
在缺血前、缺血期间和再灌注时连续记录标准心电图（三根肢体导联：I、II 和 III，以及三根计算增强导联：aVL、aVR 和 aVF）（图 2）。心电图显示心动过速、心律失常（即心室颤动）、传导系统缺陷（即束支传导阻滞）、梗死相关 Q 波的发展和 ST 段偏差<sup class="...

Watch this Scientific Journal Video about Model of Ischemia and Reperfusion Injury in Rabbits at JoVE.com

Model of Ischemia and Reperfusion Injury in Rabbits

本研究展示了兔子急性局部心肌缺血和再灌注损伤的高度可重复动物模型，使用左侧微型开胸术治疗生存病例或中线胸骨切开术治疗非生存病例。

兔子缺血和再灌注损伤模型

The protocol here provides a simple, highly replicable methodology to induce in situ acute regional myocardial ischemia in the rabbit for non-survival and ...

该协议提供了一种简单，可靠且可重复的方法，用于在兔模型中诱导原位急性区域心肌缺血和再灌注损伤，用于非生存和生存实验。该技术通过两种不同的手术方法具有最低的死亡率和发病率。该方法具有成本效益且技术要求低，因此无需手术专业知识即可由研究人员执行。通过微型开胸手术暴露 LAD 可能具有挑战性。我们建议在胸骨旁前方做第四个肋间间隙，向外侧延伸 5 到 6 厘米，并事先进行此类暴露和兔尸检。要进行左侧迷你开胸手术以进行生存研究，请使用枕头将麻醉兔的左侧抬高约 30 度。用毡尖标记笔触诊并勾勒出骨质地标。使用 10 号刀片，切开覆盖在第五根肋骨上的皮肤，从胸骨外侧缘附近延伸到横向 5 到 6 厘米。使用电烙术，将胸大肌内侧分开，然后将前锯肌外侧分开。然后将肋间肌肉分开到第五肋骨上方，以保护神经血管束。使用锋利的解剖，用 Metzenbaum 剪刀切开顶层胸膜，小心地通过第四肋间隙进入胸膜腔，并在平行于肋骨的两个方向上延伸初始胸膜切口，直到可以插入肋骨扩张器或胸骨牵开器。在肋骨间隙内放置肋骨扩张器或胸骨牵开器，并将其加宽以观察心脏和心包囊。使用 DeBakey 镊子抬起心包。用 Metzenbaum 剪刀将其打开，然后进行 LAD 动脉隔离。这将提供心脏前外侧表面的最佳可视化，并充分暴露于 LAD 及其分支。为了隔离 LAD 动脉，用锥形针上的 3-0 聚丙烯缝合线包围其第三对角线分支，然后取出针头。在生存情况下，修剪用于隔离 LAD 的三根 3-0 聚丙烯线，并将末端松散地绑在一起。三根 3-0 聚丙烯线用于在采集心脏时识别有风险的区域和梗塞区域。手术后，在肋骨周围绑上两针 2-0 Polyglactin 910 八字形缝线。用 3-0 聚二恶烷酮缝合线以跑步方式闭合肌肉层和皮下层。然后使用 5-0 单丝缝合线以皮下方式闭合皮肤。最后，进行穿刺胸腔穿刺术以清除胸膜错误。对于非生存期研究，立即使用弯曲的妙佑医疗剪刀进行中线胸骨切开术。使用胸骨牵开器观察心脏和心包囊，如前所述打开心包。可视化在先前进行的开胸手术部分放置的 3-0 聚丙烯螺纹环。为了隔离 LAD 动脉，用锥形针上的 3-0 聚丙烯缝合线包围其第三对角线分支。然后取出针，将聚丙烯长丝的两端穿过一个小乙烯基管，形成一个圈套。使用 DeBakey 镊子，在两根聚丙烯长丝之间放置一个矩形 PTFE 基料，将其夹在隔离的 LAD 动脉和乙烯基管之间。通过按下乙烯基管并向上拉聚丙烯缝合线丝以阻塞冠状动脉来收紧此圈套。夹 clamp 使用蚊子夹子保持所需的密封性。通过观察心外膜的局部发绀来确认心肌缺血。在缺血诱导 30 分钟后取下圈套器。在实验结束时，收获心脏进行生化和组织分析。在缺血前期、缺血期间和再灌注期间记录的心电图显示心动过速、心律失常、传导系统缺陷、梗死相关 Q 波的发展和 ST 段偏差。二维回波读数显示，与缺血前相比，缺血期间和缺血后缩短分数减少。诱发局部心肌缺血 30 分钟后，观察到心肌坏死的证据。最关键的方案步骤是在不损伤动脉或引起静脉出血的情况下小心地包围 LAD，适当放置 PTFE 承诺以防止血管痉挛，并包括 LAD 以创建一个一致的风险区域。该方法可用于血管损伤、慢性心肌缺血和急性心肌眩晕模型，以揭示心血管分子力学、病理生理机制和治疗靶点，证明可用性并开发诊断和治疗算法。

Research

JoVE Journal

Medicine

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

常温心脏骤停和心肺复苏术：缺血再灌注损伤的小鼠模型

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality1-4 and which is most 
frequently caused ...

科研

医学

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

急性肾损伤和伤后纤维化小鼠缺血再灌注模型

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

Murine Model of Intestinal Ischemia-reperfusion Injury

肠缺血再灌注损伤的小鼠模型

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography

小鼠使用表面垫心电图心肌缺血再灌注损伤的确认

Many animal models have been established for the study of myocardial remodeling and heart failure due to its status as the number one cause of mortality ...

Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits

糖尿病家兔欣德肢体缺血的临床前模型

Peripheral vascular disease is a widespread clinical problem that affects millions of patients worldwide. A major consequence of peripheral vascular ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

大鼠心肌缺血再灌注后冠状动脉流动储备评价

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

重建显微外科缺血再灌注损伤研究的临床前大鼠模型

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

在穆林模型中缺血再灌注损伤后干细胞的心肌内传递延迟

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

器官缺血-输液损伤模拟大鼠肝脏移植模型的血动力学变化

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...

A Rat Lung Transplantation Model of Warm Ischemia/Reperfusion Injury: Optimizations to Improve Outcomes

温缺血/再灌注损伤的大鼠肺移植模型：改善结局的优化

From our experience with rat lung transplantation, we have found several areas for improvement. Information in the existing literature regarding methods ...