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The In ovo CAM-assay as a Xenograft Model for Sarcoma

25.1K Views

12:44 min

July 17th, 2013

July 17th, 2013

25,146 Views

0:05

Title

2:19

Preparation of Tumor-derived Single Cell Suspensions

3:59

Opening of the Eggs

5:21

Inoculation Procedure

6:23

Imaging and Harvesting of the CAM

7:20

Results: Evaluation of the CAM

11:30

Conclusion

The in ovo chorioallantoic membrane (CAM) is grafted with fresh sarcoma-derived tumor tissues, their single cell suspensions, and permanent and transient fluorescently labeled established sarcoma cell lines. The model is used to study graft- (viability, Ki67 proliferation index, necrosis, infiltration) and host (fibroblast infiltration, vascular ingrowth) behavior.

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