Methods Article

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

2.7K Views

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05:22 min

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February 24th, 2023

DOI :

10.3791/64886-v

February 24th, 2023

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Kyra Harvey¹, Peter J. Madsen²^,³, Tiffany Smith², Crystal Griffin¹^,², Luke Patterson¹^,², Nicholas A. Vitanza⁴^,⁵, Phillip B. Storm²^,³, Adam C. Resnick²^,³, Jessica B. Foster¹^,²

¹Division of Oncology, Children’s Hospital of Philadelphia, ²Center for Data Driven Discovery in Biomedicine, Children’s Hospital of Philadelphia, ³Division of Neurosurgery, Children’s Hospital of Philadelphia, ⁴Ben Towne Center for Childhood Cancer Research, Seattle Children’s Research Institute, ⁵Department of Pediatrics, Seattle Children’s Hospital, University of Washington

Summary

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Intracranial Cannula

CAR T cell Therapy

Locoregional Delivery

Central Nervous System Tumors

Surgical Procedure

Stereotactic Apparatus

Neuroscience Research

Multiple Doses

Invasive Techniques

Chapters in this video

0:04

Introduction

0:48

Mouse Preparation and Surgery Procedure

3:47

CAR T Cell Infusion

4:36

Results: Efficacy of CAR T Cell Therapy

4:53

Conclusion

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